Title : Concomitant evaluation of PMA+ionomycin-induced kinase phosphorylation and cytokine production in T cell subsets by flow cytometry.

Pub. Date : 2014 Mar

PMID : 24464647






1 Functional Relationships(s)
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1 We examined intracellular ERK1/2 phosphorylation and IFN-gamma production by CD4+ and CD8+ T cells upon polyclonal stimulation with PMA and ionomycin, while monitoring expression of the cytolytic molecule perforin and the T cell activation marker CD38. Tetradecanoylphorbol Acetate interferon gamma Homo sapiens