Pub. Date : 2014
PMID : 24407515
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | MCF-7/GSK-3beta(KD) cells were more resistant to doxorubicin and tamoxifen compared with either MCF-7/GSK-3beta(WT) or MCF-7/GSK-3beta(A9) cells. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 2 | In the presence and absence of doxorubicin, the MCF-7/GSK-3beta(KD) cells formed more colonies in soft agar compared with MCF-7/GSK-3beta(WT) or MCF-7/GSK-3beta(A9) cells. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 3 | However, resistance to doxorubicin and tamoxifen were alleviated in MCF-7/GSK-3beta(KD) cells upon co-treatment with an MEK inhibitor, indicating regulation of this resistance by the Raf/MEK/ERK pathway. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 4 | Treatment of MCF-7 and MCF-7/GSK-3beta(WT) cells with doxorubicin eliminated the detection of S9-phosphorylated GSK-3beta, while total GSK-3beta was still detected. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 5 | Treatment of MCF-7 and MCF-7/GSK-3beta(WT) cells with doxorubicin eliminated the detection of S9-phosphorylated GSK-3beta, while total GSK-3beta was still detected. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 6 | Treatment of MCF-7 and MCF-7/GSK-3beta(WT) cells with doxorubicin eliminated the detection of S9-phosphorylated GSK-3beta, while total GSK-3beta was still detected. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 7 | In contrast, S9-phosphorylated GSK-3beta was still detected in MCF-7/GSK-3beta(KD) and MCF-7/GSK-3beta(A9) cells, indicating that one of the effects of doxorubicin on MCF-7 cells was suppression of S9-phosphorylated GSK-3beta, which could result in increased GSK-3beta activity. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |
| 8 | Taken together, these results demonstrate that introduction of GSK-3beta(KD) into MCF-7 breast cancer cells promotes resistance to doxorubicin and tamoxifen, but sensitizes the cells to mTORC1 blockade by rapamycin. | Doxorubicin | glycogen synthase kinase 3 beta | Homo sapiens |