Title : A High Throughput Assay for Discovery of Small Molecules that Bind AMP-activated Protein Kinase (AMPK).

Pub. Date : 2013

PMID : 24396733






1 Functional Relationships(s)
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1 To identify drugs that modulate AMPK activity independent of the canonical ATP-binding pocket found throughout the kinome, we designed a robust fluorescence-based high throughput screening assay biased toward the identification of molecules that bind the regulatory region of AMPK through displacement of MANT-ADP, a fluorescent ADP analog. Adenosine Diphosphate protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens