Title : Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation.

Pub. Date : 2013 Nov 8

PMID : 24062310






3 Functional Relationships(s)
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1 Biochemical and genetic analyses using mutant strains of Saccharomyces cerevisiae revealed that the generation of fOSs is tightly correlated with the N-glycosylation activity of OST. Nitrogen dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Homo sapiens
2 Furthermore, we present evidence that the purified OST complex can generate fOSs by hydrolyzing dolichol-linked oligosaccharide, the glycan donor substrate for N-glycosylation. Nitrogen dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Homo sapiens
3 The heterologous expression of a single subunit of OST from the protozoan Leishmania major in S. cerevisiae demonstrated that this enzyme functions both in N-glycosylation and generation of fOSs. Nitrogen dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Homo sapiens