Title : A western blot assay to measure cyclin dependent kinase activity in cells or in vitro without the use of radioisotopes.

Pub. Date : 2013 Sep 17

PMID : 23954627






1 Functional Relationships(s)
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1 By combining this substrate in a western blot method using fluorochrome based antibodies and phospho-imager analysis, we measured the Km of ATP binding to Cdk1 to be 3.5 muM. Adenosine Triphosphate cyclin dependent kinase 1 Homo sapiens