Pub. Date : 2013 Jun
PMID : 23530057
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We previously reported the structure of an autoinhibited ZAP-70 variant in which two regulatory tyrosine residues (315 and 319) in the SH2-kinase linker were replaced by phenylalanine. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |
| 2 | We now present a crystal structure of ZAP-70 in which Tyr 315 and Tyr 319 are not mutated, leading to the recognition of a five-residue sequence register error in the SH2-kinase linker of the original crystallographic model. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |
| 3 | We now present a crystal structure of ZAP-70 in which Tyr 315 and Tyr 319 are not mutated, leading to the recognition of a five-residue sequence register error in the SH2-kinase linker of the original crystallographic model. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |
| 4 | As seen in a recently reported structure of the related tyrosine kinase Syk, Tyr 315 of ZAP-70 is part of a hydrophobic interface between the regulatory apparatus and the kinase domain, and the integrity of this interface would be lost upon engagement of doubly phosphorylated peptides by the SH2 domains. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |
| 5 | Tyr 319 is not necessarily dislodged by SH2 engagement, which activates ZAP-70 only ~5-fold in vitro. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |
| 6 | This difference is due to the ability of Tyr 319 to suppress ZAP-70 activity even when the SH2 domains are dislodged from the kinase domain, providing stringent control of ZAP-70 activity downstream of Lck. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |
| 7 | This difference is due to the ability of Tyr 319 to suppress ZAP-70 activity even when the SH2 domains are dislodged from the kinase domain, providing stringent control of ZAP-70 activity downstream of Lck. | Tyrosine | zeta chain of T cell receptor associated protein kinase 70 | Homo sapiens |