Pub. Date : 2012
PMID : 23093908
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Bid-overexpression regulates proliferation and phosphorylation of Akt and MAPKs in response to etoposide-induced DNA damage in hepatocellular carcinoma cells. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 2 | However, the effects of Bid on hepatocellular carcinoma (HCC) cell proliferation in response to etoposide-induced DNA damage have not been sufficiently investigated. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 3 | METHODS: Using a stable Bid-overexpression HCC cell line, Bid/PLC/PRF/5, overexpression of Bid promoted loss of viability in response to etoposide-induced DNA damage. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 4 | METHODS: Using a stable Bid-overexpression HCC cell line, Bid/PLC/PRF/5, overexpression of Bid promoted loss of viability in response to etoposide-induced DNA damage. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 5 | METHODS: Using a stable Bid-overexpression HCC cell line, Bid/PLC/PRF/5, overexpression of Bid promoted loss of viability in response to etoposide-induced DNA damage. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 6 | RESULTS: The survival rates of 100 muM etoposide on the cells with control vector and Bid/PLC/PRF/5 at 48 hours amounted to 71% +- 0.75% and 59% +- 0.60% with MTT assay, and similar results of 85% +- 0.08% and 63% +- 0.14% with BrdU-labeling assay respectively. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 7 | Moreover, overexpression of Bid sensitized the cells to apoptosis at a high dose of etoposide (causing irreparable damage). | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 8 | Overexpression of Bid suppressed the activation of Akt with respect to etoposide-induced DNA damage. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 9 | CONCLUSION: Taken together, these results suggest that overexpression of Bid suppressed the activation of Akt, p38, and c-Jun, and promoted the activation of ERK1/2 induced by etoposide, suggesting that the promotion of ERK1/2 activation may have a negative effect on Bid-mediated HCC DNA damage induced by etoposide. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 10 | CONCLUSION: Taken together, these results suggest that overexpression of Bid suppressed the activation of Akt, p38, and c-Jun, and promoted the activation of ERK1/2 induced by etoposide, suggesting that the promotion of ERK1/2 activation may have a negative effect on Bid-mediated HCC DNA damage induced by etoposide. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |
| 11 | CONCLUSION: Taken together, these results suggest that overexpression of Bid suppressed the activation of Akt, p38, and c-Jun, and promoted the activation of ERK1/2 induced by etoposide, suggesting that the promotion of ERK1/2 activation may have a negative effect on Bid-mediated HCC DNA damage induced by etoposide. | Etoposide | BH3 interacting domain death agonist | Homo sapiens |