Title : Cellular recovery of glyceraldehyde-3-phosphate dehydrogenase activity and thiol status after exposure to hydroperoxides.

Pub. Date : 1990 Jan

PMID : 2297224






8 Functional Relationships(s)
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1 The activity of the thiol-dependent enzyme glyceraldehyde-3-phosphate dehydrogenase (GPD), in vertebrate cells, was modulated by a change in the intracellular thiol:disulfide redox status. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
2 The activity of the thiol-dependent enzyme glyceraldehyde-3-phosphate dehydrogenase (GPD), in vertebrate cells, was modulated by a change in the intracellular thiol:disulfide redox status. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
3 The activity of the thiol-dependent enzyme glyceraldehyde-3-phosphate dehydrogenase (GPD), in vertebrate cells, was modulated by a change in the intracellular thiol:disulfide redox status. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
4 The activity of the thiol-dependent enzyme glyceraldehyde-3-phosphate dehydrogenase (GPD), in vertebrate cells, was modulated by a change in the intracellular thiol:disulfide redox status. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
5 Loss of reduced protein thiols, as measured by binding of the thiol reagent iodoacetic acid to GPD, and loss of GPD enzymatic activity occurred in a dose-dependent manner. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
6 Loss of reduced protein thiols, as measured by binding of the thiol reagent iodoacetic acid to GPD, and loss of GPD enzymatic activity occurred in a dose-dependent manner. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
7 The enzymatic recovery of GPD activity was observed either without addition of thiols to the medium or by incubation of a sonicated cell mixture with 2 mM cysteine, cystine, cysteamine, or glutathione (GSH); GSSG had no effect. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens
8 These findings indicate that the cellular thiol redox status can be important in determining GPD enzymatic activity. Sulfhydryl Compounds glyceraldehyde-3-phosphate dehydrogenase Homo sapiens