Title : AMPK enhances insulin-stimulated GLUT4 regulation via lowering membrane cholesterol.

Pub. Date : 2012 May

PMID : 22434076






2 Functional Relationships(s)
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1 Using L6 myotubes stably expressing an exofacial myc-epitope-tagged-GLUT4, AMPK stimulation by 5-aminoimidazole-4-carboxamide-1-beta-d-ribonucleoside (AICAR; 45 min, 1 mm) or 2,4-dinitrophenol (DNP; 30 min, 200 mum) increased cell surface GLUT4myc labeling by approximately 25% (P < 0.05). acadesine solute carrier family 2 member 4 Rattus norvegicus
2 Whereas AMPK knockdown prevented the enhanced basal and insulin-stimulated GLUT4myc labeling by AICAR and DNP, cholesterol replenishment only blocked the AMPK-associated enhancement in insulin action. acadesine solute carrier family 2 member 4 Rattus norvegicus