Title : Conformational changes of α-lactalbumin adsorbed at oil-water interfaces: interplay between protein structure and emulsion stability.

Pub. Date : 2012 Feb 7

PMID : 22201548






7 Functional Relationships(s)
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1 Conformational changes of alpha-lactalbumin adsorbed at oil-water interfaces: interplay between protein structure and emulsion stability. Water lactalbumin alpha Homo sapiens
2 The conformation and structural dimensions of alpha-lactalbumin (alpha-La) both in solution and adsorbed at oil-water interfaces of emulsions were investigated using synchrotron radiation circular dichroism (SRCD) spectroscopy, front-face tryptophan fluorescence (FFTF) spectroscopy, and dual polarization interferometry (DPI). Water lactalbumin alpha Homo sapiens
3 The conformation and structural dimensions of alpha-lactalbumin (alpha-La) both in solution and adsorbed at oil-water interfaces of emulsions were investigated using synchrotron radiation circular dichroism (SRCD) spectroscopy, front-face tryptophan fluorescence (FFTF) spectroscopy, and dual polarization interferometry (DPI). Water lactalbumin alpha Homo sapiens
4 The far-UV SRCD results showed that adsorption of alpha-La at oil-water interfaces created a new non-native secondary structure that was more stable to thermally induced conformational changes. Water lactalbumin alpha Homo sapiens
5 The DPI study showed that adsorption of alpha-La to a hydrophobic C18-water surface caused a change in the dimensions of alpha-La from the native globule-like shape (2.5-3.7 nm) to a compact/dense layer approximately 1.1 nm thick. Water lactalbumin alpha Homo sapiens
6 The DPI study showed that adsorption of alpha-La to a hydrophobic C18-water surface caused a change in the dimensions of alpha-La from the native globule-like shape (2.5-3.7 nm) to a compact/dense layer approximately 1.1 nm thick. Water lactalbumin alpha Homo sapiens
7 This study reports new information on the secondary and tertiary structural changes of alpha-La upon adsorption to oil-water interfaces. Water lactalbumin alpha Homo sapiens