Title : Protein isoaspartate methyltransferase-mediated 18O-labeling of isoaspartic acid for mass spectrometry analysis.

Pub. Date : 2012 Jan 17

PMID : 22132761






1 Functional Relationships(s)
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1 Here we report a novel assay for Asp isomerization by isotopic labeling with (18)O via a two-step process: the isoAsp peptide is first specifically methylated by protein isoaspartate methyltransferase (PIMT, EC 2.1.1.77) to the corresponding methyl ester, which is subsequently hydrolyzed in (18)O-water to regenerate isoAsp. Aspartic Acid protein-L-isoaspartate (D-aspartate) O-methyltransferase Homo sapiens