Title : An E. coli expression system for the rapid purification and characterization of a v-abl tyrosine protein kinase.

Pub. Date : 1990

PMID : 2157186






1 Functional Relationships(s)
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1 A bacterial expression vector containing a segment of the v-abl gene from Abelson murine leukemia virus (A-MuLV) was constructed such that the gag region of v-abl was replaced by a sequence encoding the IgG-binding domain of the S. aureus protein A. pabl HP, a fusion protein encoded by this vector was rapidly purified to near homogeneity by affinity chromatography on IgG-Affigel and Mono Q FPLC. Glycosaminoglycans ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens