Title : RNAi-mediated down-regulation of ornithine decarboxylase (ODC) leads to reduced nicotine and increased anatabine levels in transgenic Nicotiana tabacum L.

Pub. Date : 2011 Apr

PMID : 21232776






8 Functional Relationships(s)
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1 RNAi-mediated down-regulation of ornithine decarboxylase (ODC) leads to reduced nicotine and increased anatabine levels in transgenic Nicotiana tabacum L. In leaf and root tissues of Nicotiana tabacum L. (common tobacco), nicotine is by far the predominant pyridine alkaloid, with anatabine representing only a minor component of the total alkaloid fraction. Nicotine ornithine decarboxylase Nicotiana tabacum
2 RNAi-mediated down-regulation of ornithine decarboxylase (ODC) leads to reduced nicotine and increased anatabine levels in transgenic Nicotiana tabacum L. In leaf and root tissues of Nicotiana tabacum L. (common tobacco), nicotine is by far the predominant pyridine alkaloid, with anatabine representing only a minor component of the total alkaloid fraction. Nicotine ornithine decarboxylase Nicotiana tabacum
3 RNAi-mediated down-regulation of ornithine decarboxylase (ODC) leads to reduced nicotine and increased anatabine levels in transgenic Nicotiana tabacum L. In leaf and root tissues of Nicotiana tabacum L. (common tobacco), nicotine is by far the predominant pyridine alkaloid, with anatabine representing only a minor component of the total alkaloid fraction. Nicotine ornithine decarboxylase Nicotiana tabacum
4 RNAi-mediated down-regulation of ornithine decarboxylase (ODC) leads to reduced nicotine and increased anatabine levels in transgenic Nicotiana tabacum L. In leaf and root tissues of Nicotiana tabacum L. (common tobacco), nicotine is by far the predominant pyridine alkaloid, with anatabine representing only a minor component of the total alkaloid fraction. Nicotine ornithine decarboxylase Nicotiana tabacum
5 The pyrrolidine ring of nicotine is derived from the diamine putrescine, which can be synthesized either directly from ornithine via the action of ODC, or from arginine via a three enzymatic step process, initiated by ADC. Nicotine ornithine decarboxylase Nicotiana tabacum
6 We observed a marked effect upon the alkaloid profile of transgenic tissues, with ODC transcript down-regulation leading to reduced nicotine and increased anatabine levels in both cultured hairy roots and intact greenhouse-grown plants. Nicotine ornithine decarboxylase Nicotiana tabacum
7 We conclude that the ODC mediated route to putrescine plays an important role in determining the normal nicotine:anatabine profile in N. tabacum and is essential in allowing N. tabacum to increase nicotine levels in response to wound-associated stress. Nicotine ornithine decarboxylase Nicotiana tabacum
8 We conclude that the ODC mediated route to putrescine plays an important role in determining the normal nicotine:anatabine profile in N. tabacum and is essential in allowing N. tabacum to increase nicotine levels in response to wound-associated stress. Nicotine ornithine decarboxylase Nicotiana tabacum