Title : Activation of the regulator of G protein signaling 14-Gαi1-GDP signaling complex is regulated by resistance to inhibitors of cholinesterase-8A.

Pub. Date : 2011 Feb 8

PMID : 21158412






5 Functional Relationships(s)
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1 Unlike other RGS proteins, RGS14 also contains a G protein regulatory (also known as GoLoco) domain that binds Galphai1/3-GDP in cells and in vitro. Guanosine Diphosphate regulator of G protein signaling 14 Homo sapiens
2 Here we report that Ric-8A, a nonreceptor guanine nucleotide exchange factor (GEF), functionally interacts with the RGS14-Galphai1-GDP signaling complex to regulate its activation state. Guanosine Diphosphate regulator of G protein signaling 14 Homo sapiens
3 Consistent with this idea, Ric-8A stimulates dissociation of the RGS14-Galphai1-GDP complex in cells and in vitro using purified proteins. Guanosine Diphosphate regulator of G protein signaling 14 Homo sapiens
4 Purified Ric-8A stimulates dissociation of the RGS14-Galphai1-GDP complex to form a stable Ric-8A-Galphai complex in the absence of GTP. Guanosine Diphosphate regulator of G protein signaling 14 Homo sapiens
5 In the presence of an activating nucleotide, Ric-8A interacts with the RGS14-Galphai1-GDP complex to stimulate both the steady-state GTPase activity of Galphai1 and binding of GTP to Galphai1. Guanosine Diphosphate regulator of G protein signaling 14 Homo sapiens