Title : Mass spectrometry detection of histidine phosphorylation on NM23-H1.

Pub. Date : 2011 Feb 4

PMID : 21121676






4 Functional Relationships(s)
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1 Mass spectrometry detection of histidine phosphorylation on NM23-H1. Histidine NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens
2 Here, we present a novel buffer system to show histidine phosphorylation of NM23-H1, the product of the first identified putative human metastasis suppressor gene (NME1), which catalyzes the transfer of the gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates. Histidine NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens
3 Here, we present a novel buffer system to show histidine phosphorylation of NM23-H1, the product of the first identified putative human metastasis suppressor gene (NME1), which catalyzes the transfer of the gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates. Histidine NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens
4 On the basis of a pH titration of LC elution buffers and MS/MS identification, recombinant NM23-H1 subjected to autophosphorylation was shown to contain phosphorylated histidine at residue 118 at pH 5 and 6, with each level giving over 75% peptide coverage for identification. Histidine NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens