Title : Purification of recombinant acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) from H293 cells and binding studies between the enzyme and substrates using difference intrinsic fluorescence spectroscopy.

Pub. Date : 2010 Nov 23

PMID : 20964445






1 Functional Relationships(s)
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Protein Name
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1 The results show that oleoyl-CoA binds to ACAT1 with K(d) = 1.9 muM and elicits significant structural changes of the protein as manifested by the significantly positive changes in its fluorescence spectrum; stearoyl-CoA elicits a similar spectrum change but much lower in magnitude. stearoyl-coenzyme A acetyl-CoA acetyltransferase 1 Homo sapiens