Title : Evaluation of alternatives for human lysozyme purification from transgenic rice: impact of phytic acid and buffer.

Pub. Date : 2010 Sep-Oct

PMID : 20945487






1 Functional Relationships(s)
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1 Using tris buffer to adjust pH 4.5 extract to pH 6 before adsorption reduced phytic acid interference by minimizing phytic acid-lysozyme interactions, eliminated the need for phytase treatment, and increased the binding capacity to 25 mg/mL. Phytic Acid prolyl 4-hydroxylase, transmembrane Homo sapiens