Pub. Date : 1991 Jul 5
PMID : 2061301
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | T-cell antigen receptor ligation induces tyrosine phosphorylation of phospholipase C-gamma 1. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 2 | Although the mechanism by which TCR ligation regulates PLC activity is unknown, recent studies suggest that coupling of this receptor complex to PLC activity is dependent on an intermediate protein tyrosine phosphorylation event(s). | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 3 | In the present study, we demonstrate that antibody-mediated TCR cross-linkage results in the tyrosine phosphorylation of PLC-gamma 1. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 4 | Stimulation of the TCR for 30 s induced a 4-5-fold increase in the level of PLC activity recovered in anti-phosphotyrosine (Tyr(P)) antibody immunoprecipitates from stimulated Jurkat cells. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 5 | Furthermore, the TCR-mediated increase in anti-Tyr(P) antibody-bound PLC activity was inhibited by staurosporine at drug concentrations identical with those required for in vivo inhibition of TCR-dependent phosphoinositide breakdown. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 6 | Furthermore, the TCR-mediated increase in anti-Tyr(P) antibody-bound PLC activity was inhibited by staurosporine at drug concentrations identical with those required for in vivo inhibition of TCR-dependent phosphoinositide breakdown. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 7 | Immunoblot analyses demonstrated that TCR ligation dramatically increased the level of tyrosine-phosphorylated PLC-gamma 1 present in anti-Tyr(P) antibody immunoprecipitates from stimulated Jurkat cells. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 8 | Immunoblot analyses demonstrated that TCR ligation dramatically increased the level of tyrosine-phosphorylated PLC-gamma 1 present in anti-Tyr(P) antibody immunoprecipitates from stimulated Jurkat cells. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |
| 9 | These results strongly suggest that the TCR complex expressed by Jurkat cells is functionally coupled to the phosphoinositide-dependent signaling pathway through the tyrosine phosphorylation of PLC-gamma 1. | Tyrosine | T cell receptor beta variable 20/OR9-2 (non-functional) | Homo sapiens |