Title : A proton-shuttle reaction mechanism for histone deacetylase 8 and the catalytic role of metal ions.

Pub. Date : 2010 Jul 14

PMID : 20568751






2 Functional Relationships(s)
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1 Instead, our results indicated that HDAC8 employs a proton-shuttle catalytic mechanism, in which a neutral His143 first serves as the general base to accept a proton from the zinc-bound water molecule in the initial rate-determining nucleophilic attack step, and then shuttles it to the amide nitrogen atom to facilitate the cleavage of the amide bond. Amides histone deacetylase 8 Homo sapiens
2 Instead, our results indicated that HDAC8 employs a proton-shuttle catalytic mechanism, in which a neutral His143 first serves as the general base to accept a proton from the zinc-bound water molecule in the initial rate-determining nucleophilic attack step, and then shuttles it to the amide nitrogen atom to facilitate the cleavage of the amide bond. Amides histone deacetylase 8 Homo sapiens