Pub. Date : 2010 Jan 29
PMID : 19940145
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells. | Glucose | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 2 | Previously, we have demonstrated that elevated glucose levels induce GAPDH nuclear accumulation in retinal Muller cells. | Glucose | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 3 | Therefore, this study investigated the role of siah-1 in high glucose-induced GAPDH nuclear translocation and subsequent cell death in retinal Muller cells. | Glucose | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 4 | High glucose significantly increased siah-1 expression within 12 h. Under hyperglycemic conditions, siah-1 formed a complex with GAPDH and was predominantly localized in the nucleus of Muller cells. | Glucose | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 5 | siah-1 knockdown using 50 nm siah-1 small interfering RNA significantly decreased high glucose-induced GAPDH nuclear accumulation at 24 h by 43.8 +/- 4.0%. | Glucose | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 6 | Further, knockdown of siah-1 prevented high glucose-induced cell death of Muller cells potentially by inhibiting p53 phosphorylation consistent with previous observations, indicating that nuclear GAPDH induces cell death via p53 activation. | Glucose | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |