Pub. Date : 1990 Aug
PMID : 1973454
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Glutamate-dependent active-site labeling of brain glutamate decarboxylase. | Glutamic Acid | glutamate-ammonia ligase | Rattus norvegicus |
| 2 | To test this prediction, the effect of glutamate on the incorporation of pyridoxal-P into rat-brain GAD was studied by incubating GAD with [32P]pyridoxal-P, followed by reduction with NaBH4 to link irreversibly the cofactor to the enzyme. | Glutamic Acid | glutamate-ammonia ligase | Rattus norvegicus |
| 3 | Adding glutamate to the reaction mixture strongly stimulated labeling of GAD, as expected. | Glutamic Acid | glutamate-ammonia ligase | Rattus norvegicus |
| 4 | 4-Deoxypyridoxine 5"-phosphate (deoxypyridoxine-P), a close structural analogue of pyridoxal-P, was a competitive inhibitor of the activation of glutamate apodecarboxylase by pyridoxal-P (Ki = 0.27 microM) and strongly inhibited glutamate-dependent labeling of GAD. | Glutamic Acid | glutamate-ammonia ligase | Rattus norvegicus |
| 5 | Both proteins could be purified by immunoaffinity chromatography on a column prepared with a monoclonal antibody to GAD, and both were labeled in a glutamate-dependent, deoxypyridoxine-P-sensitive manner, indicating that both were GAD. | Glutamic Acid | glutamate-ammonia ligase | Rattus norvegicus |
| 6 | Both proteins could be purified by immunoaffinity chromatography on a column prepared with a monoclonal antibody to GAD, and both were labeled in a glutamate-dependent, deoxypyridoxine-P-sensitive manner, indicating that both were GAD. | Glutamic Acid | glutamate-ammonia ligase | Rattus norvegicus |