Title : Homo-oligomerization and activation of AMP-activated protein kinase are mediated by the kinase domain alphaG-helix.

Pub. Date : 2009 Oct 2

PMID : 19651772






1 Functional Relationships(s)
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1 Mutation of the corresponding AMPK alpha-subunit residues (Val-219 and Phe-223) to glutamate reduced the tendency of the kinase to form higher order homo-oligomers, as was determined by the following three independent techniques in vitro: (i) small angle x-ray scattering, (ii) surface plasmon resonance spectroscopy, and (iii) two-dimensional blue native/SDS-PAGE. Glutamic Acid protein kinase AMP-activated catalytic subunit alpha 1 Homo sapiens