Title : Silencing neuroglobin enhances neuronal vulnerability to oxidative injury by down-regulating 14-3-3gamma.

Pub. Date : 2009 Jul

PMID : 19574997






4 Functional Relationships(s)
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1 A WST-8 assay demonstrated that viability was significantly decreased in N2a/Ngb-siRNA cells and N2a cells transiently transfected with Ngb-siRNA plasmids compared with controls following hydrogen peroxide treatment. Hydrogen Peroxide neuroglobin Mus musculus
2 An LDH assay demonstrated a time-dependent increase in the death of Ngb-siRNA-transfected N2a cells following hydrogen peroxide treatment. Hydrogen Peroxide neuroglobin Mus musculus
3 Hoechst staining demonstrated that the quantity of apoptotic cells among N2a/Ngb-siRNA cells following hydrogen peroxide treatment significantly increased compared with controls. Hydrogen Peroxide neuroglobin Mus musculus
4 Transfection of 14-3-3gamma plasmids significantly enhanced the viability of N2a/Ngb-siRNA cells following hydrogen peroxide treatment compared with vector controls. Hydrogen Peroxide neuroglobin Mus musculus