Title : Sensitive fluorimetric assay for serum angiotensin-converting enzyme with the natural substrate angiotensin I.

Pub. Date : 1977 Aug

PMID : 195458






2 Functional Relationships(s)
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1 Angiotensin I (0.2 mM in 0.1 M potassium phosphate-30 mM NaCl, pH 7.5; 37 C) is converted to angiotensin II and L-histidyl-L-leucine, which is quantified fluorimetrically (excitation = 360 nm; fluorescence, 500 nm) by formation of a fluorescent adduct with O-phthaldialdehyde. Sodium Chloride angiotensinogen Homo sapiens
2 Angiotensin I (0.2 mM in 0.1 M potassium phosphate-30 mM NaCl, pH 7.5; 37 C) is converted to angiotensin II and L-histidyl-L-leucine, which is quantified fluorimetrically (excitation = 360 nm; fluorescence, 500 nm) by formation of a fluorescent adduct with O-phthaldialdehyde. Sodium Chloride angiotensinogen Homo sapiens