Pub. Date : 2008 May
PMID : 18287519
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA. | Nocodazole | Rho/Rac guanine nucleotide exchange factor 2 | Homo sapiens |
| 2 | The overexpression of GEF-H1 deficient in microtubule binding or treatment of HeLa cells with nocodazole to induce microtubule depolymerization results in Rho-dependent actin stress fiber formation and contractile cell morphology. | Nocodazole | Rho/Rac guanine nucleotide exchange factor 2 | Homo sapiens |
| 3 | However, whether GEF-H1 is required and sufficient to mediate nocodazole-induced contractility remains unclear. | Nocodazole | Rho/Rac guanine nucleotide exchange factor 2 | Homo sapiens |
| 4 | We establish here that siRNA-mediated depletion of GEF-H1 in HeLa cells prevents nocodazole-induced cell contraction. | Nocodazole | Rho/Rac guanine nucleotide exchange factor 2 | Homo sapiens |
| 5 | Furthermore, the nocodazole-induced activation of RhoA and Rho-associated kinase (ROCK) that mediates phosphorylation of myosin regulatory light chain (MLC) is impaired in GEF-H1-depleted cells. | Nocodazole | Rho/Rac guanine nucleotide exchange factor 2 | Homo sapiens |
| 6 | Our studies reveal a critical role for a GEF-H1/RhoA/ROCK/MLC signaling pathway in mediating nocodazole-induced cell contractility. | Nocodazole | Rho/Rac guanine nucleotide exchange factor 2 | Homo sapiens |