Title : Distinct energetics and closing pathways for DNA polymerase beta with 8-oxoG template and different incoming nucleotides.

Pub. Date : 2007 Feb 21

PMID : 17313689






5 Functional Relationships(s)
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1 To interpret in atomic and energetic detail how pol beta processes 8-oxoG, we apply transition path sampling to delineate closing pathways of pol beta 8-oxoG complexes with dCTP and dATP incoming nucleotides and compare the results to those of the nonlesioned G:dCTP and G:dATPanalogues. 2'-deoxycytidine 5'-triphosphate DNA polymerase beta Homo sapiens
2 To interpret in atomic and energetic detail how pol beta processes 8-oxoG, we apply transition path sampling to delineate closing pathways of pol beta 8-oxoG complexes with dCTP and dATP incoming nucleotides and compare the results to those of the nonlesioned G:dCTP and G:dATPanalogues. 2'-deoxycytidine 5'-triphosphate DNA polymerase beta Homo sapiens
3 To interpret in atomic and energetic detail how pol beta processes 8-oxoG, we apply transition path sampling to delineate closing pathways of pol beta 8-oxoG complexes with dCTP and dATP incoming nucleotides and compare the results to those of the nonlesioned G:dCTP and G:dATPanalogues. 2'-deoxycytidine 5'-triphosphate DNA polymerase beta Homo sapiens
4 To interpret in atomic and energetic detail how pol beta processes 8-oxoG, we apply transition path sampling to delineate closing pathways of pol beta 8-oxoG complexes with dCTP and dATP incoming nucleotides and compare the results to those of the nonlesioned G:dCTP and G:dATPanalogues. 2'-deoxycytidine 5'-triphosphate DNA polymerase beta Homo sapiens
5 CONCLUSION: These results suggest that the lower insertion efficiency (larger Km) for dATP compared to dCTP opposite 8-oxoG is caused by a less stable closed-form of pol beta, destabilized by unfavorable interactions between Tyr271 and the mispair. 2'-deoxycytidine 5'-triphosphate DNA polymerase beta Homo sapiens