Title : Downregulation of Kir4.1 inward rectifying potassium channel subunits by RNAi impairs potassium transfer and glutamate uptake by cultured cortical astrocytes.

Pub. Date : 2007 Feb

PMID : 17091490






4 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Downregulation of Kir4.1 inward rectifying potassium channel subunits by RNAi impairs potassium transfer and glutamate uptake by cultured cortical astrocytes. Potassium potassium inwardly-rectifying channel, subfamily J, member 10 Rattus norvegicus
2 The purpose of the present study was to assess the role of the inward-rectifying K+ channel subunit Kir4.1 on potassium fluxes, glutamate uptake and membrane potential in cultured rat cortical astrocytes using RNAi, whole-cell patch clamp and a colorimetric assay. Potassium potassium inwardly-rectifying channel, subfamily J, member 10 Rattus norvegicus
3 The ability of Kir4.1-suppressed cells to mediate transmembrane potassium flow, as measured by the current response to voltage ramps or sequential application of different extracellular [K+], was dramatically impaired. Potassium potassium inwardly-rectifying channel, subfamily J, member 10 Rattus norvegicus
4 Together, these data indicate that Kir4.1 channels are primarily responsible for significant hyperpolarization of cortical astrocytes and are likely to play a major role in potassium buffering. Potassium potassium inwardly-rectifying channel, subfamily J, member 10 Rattus norvegicus