Title : Sulforaphane sensitizes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-resistant hepatoma cells to TRAIL-induced apoptosis through reactive oxygen species-mediated up-regulation of DR5.

Pub. Date : 2006 Feb 1

PMID : 16452234






5 Functional Relationships(s)
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1 Sulforaphane sensitizes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-resistant hepatoma cells to TRAIL-induced apoptosis through reactive oxygen species-mediated up-regulation of DR5. sulforaphane TNF receptor superfamily member 10b Homo sapiens
2 We found that sulforaphane treatment significantly up-regulated mRNA and protein levels of DR5, a death receptor of TRAIL. sulforaphane TNF receptor superfamily member 10b Homo sapiens
3 Pretreatment with N-acetyl-l-cysteine and overexpression of catalase inhibited sulforaphane-induced up-regulation of DR5 and almost completely blocked the cotreatment-induced apoptosis. sulforaphane TNF receptor superfamily member 10b Homo sapiens
4 Furthermore, the sulforaphane-mediated sensitization to TRAIL was efficiently reduced by administration of a blocking antibody or small interfering RNAs for DR5. sulforaphane TNF receptor superfamily member 10b Homo sapiens
5 These results collectively indicate that sulforaphane-induced generation of ROS and the subsequent up-regulation of DR5 are critical for triggering and amplifying TRAIL-induced apoptotic signaling. sulforaphane TNF receptor superfamily member 10b Homo sapiens