Title : [Cloning of human beta-microglobulin gene and its high expression in Escherichia coli].

Pub. Date : 2004 Jan

PMID : 16108498






3 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 The prokaryotic expression vector containing a gene encoding mature beta2m was constructed by inserting the DNA fragment, which was generated by PCR reaction with the cloned beta2m gene as template, into an IPTG-inducible expression vector pET-3c plasmid. Isopropyl Thiogalactoside beta-2-microglobulin Homo sapiens
2 High-yield expression of beta2m was achieved in E. coli transformed with the expression vector, and most of the recombinant beta2m existed in the inclusion body after IPTG induction. Isopropyl Thiogalactoside beta-2-microglobulin Homo sapiens
3 High-yield expression of beta2m was achieved in E. coli transformed with the expression vector, and most of the recombinant beta2m existed in the inclusion body after IPTG induction. Isopropyl Thiogalactoside beta-2-microglobulin Homo sapiens