Pub. Date : 2005 Nov
PMID : 16051920
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Activation of p-ERK1/2 by nicotine in pancreatic tumor cell line AR42J: effects on proliferation and secretion. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |
| 2 | The objectives of the present study were to determine the effect of nicotine on MAPK signaling and on the proliferation of AR42J cells as well as to assess the relationship between MAPK activation and exocrine secretion in these cells. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |
| 3 | AR42J cells were incubated with nicotine and analyzed for the activation of MAPK by Western blot analysis using their respective antibodies and confirmed by immunohistochemistry. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |
| 4 | Nicotine at a dose of 100 microM induced phospho-ERK1/2 activation maximally in 3 min compared with untreated cells. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |
| 5 | Furthermore, immunofluorescence study confirmed the nicotine-induced increase in translocation of phospho-ERK1/2 to the nucleus. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |
| 6 | We conclude that nicotine treatment induced activation of ERK1/2 and increased the proliferation of AR42J cells. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |
| 7 | The data further indicate that MAPK signaling by nicotine is independent of the secretory response. | Nicotine | mitogen activated protein kinase 3 | Rattus norvegicus |