Pub. Date : 2005 Aug 12
PMID : 15951442
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Key role of conserved histidines in recombinant mouse beta-carotene 15,15"-monooxygenase-1 activity. | Histidine | beta-carotene oxygenase 1 | Mus musculus |
| 2 | Alignment of sequences of vertebrate beta-carotene 15,15"-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1). | Histidine | beta-carotene oxygenase 1 | Mus musculus |
| 3 | Alignment of sequences of vertebrate beta-carotene 15,15"-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1). | Histidine | beta-carotene oxygenase 1 | Mus musculus |
| 4 | To test this hypothesis, we produced mutant forms of mouse BCMO1 by replacing the conserved histidines and acidic residues as well as four histidines and one glutamate non-conserved in the overall family with alanines by site-directed mutagenesis. | Histidine | beta-carotene oxygenase 1 | Mus musculus |
| 5 | Therefore, the conserved histidines and Glu405 are absolutely required for the catalytic mechanism of BCMO1. | Histidine | beta-carotene oxygenase 1 | Mus musculus |