PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
15951442-0	2005	Key role of conserved histidines in recombinant mouse beta-carotene 15,15"-monooxygenase-1 activity.	Histidine	22-32	beta-carotene oxygenase 1	Mus musculus	54-90	
15951442-1	2005	Alignment of sequences of vertebrate beta-carotene 15,15"-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1).	Histidine	139-149	beta-carotene oxygenase 1	Mus musculus	37-73	
15951442-1	2005	Alignment of sequences of vertebrate beta-carotene 15,15"-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1).	Histidine	139-149	beta-carotene oxygenase 1	Mus musculus	75-80	
15951442-3	2005	To test this hypothesis, we produced mutant forms of mouse BCMO1 by replacing the conserved histidines and acidic residues as well as four histidines and one glutamate non-conserved in the overall family with alanines by site-directed mutagenesis.	Histidine	92-102	beta-carotene oxygenase 1	Mus musculus	59-64	
15951442-8	2005	Therefore, the conserved histidines and Glu405 are absolutely required for the catalytic mechanism of BCMO1.	Histidine	25-35	beta-carotene oxygenase 1	Mus musculus	102-107