Pub. Date : 2005 Mar
PMID : 15777089
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Here, we investigated the effect of bicarbonate anion (HCO3-) on the peroxidase activity stimulated by the thiol oxidase activity of copper, zinc superoxide dismutase (SOD1) using electron spin resonance (ESR) and optical techniques. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 2 | Here, we investigated the effect of bicarbonate anion (HCO3-) on the peroxidase activity stimulated by the thiol oxidase activity of copper, zinc superoxide dismutase (SOD1) using electron spin resonance (ESR) and optical techniques. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 3 | The addition of HCO3- to aerobic incubations containing SOD1, Cys, and DTPA in phosphate buffer enhanced the peroxidase activity of SOD1, as measured by hydroxylation of cyclic nitrone spin traps, dichlorodihydrofluorescein oxidation to dichlorofluorescein, and oxidation of tyrosine to dityrosine. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 4 | The addition of HCO3- to aerobic incubations containing SOD1, Cys, and DTPA in phosphate buffer enhanced the peroxidase activity of SOD1, as measured by hydroxylation of cyclic nitrone spin traps, dichlorodihydrofluorescein oxidation to dichlorofluorescein, and oxidation of tyrosine to dityrosine. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 5 | The addition of catalase inhibited the SOD1 peroxidase activity stimulated by the thiol oxidase actvity, implicating an intermediary role for H2O2 in SOD1/Cys/HCO3(-)-mediated oxidation and hydroxylation reactions. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 6 | Using a competitive kinetic method, rate constants for the reaction between the oxidant formed in the SOD1/Cys/HCO3- system and selected inhibitors were measured. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 7 | On the basis of these rate constants, we conclude that the thiol oxidase activity of SOD1 stimulates carbonate anion radical (CO3*-) formation in the presence of HCO3- and that the CO3*- formed in the SOD1/Cys/ HCO3- system is responsible for oxidation and hydroxylation reactions. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 8 | On the basis of these rate constants, we conclude that the thiol oxidase activity of SOD1 stimulates carbonate anion radical (CO3*-) formation in the presence of HCO3- and that the CO3*- formed in the SOD1/Cys/ HCO3- system is responsible for oxidation and hydroxylation reactions. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |
| 9 | On the basis of these rate constants, we conclude that the thiol oxidase activity of SOD1 stimulates carbonate anion radical (CO3*-) formation in the presence of HCO3- and that the CO3*- formed in the SOD1/Cys/ HCO3- system is responsible for oxidation and hydroxylation reactions. | Bicarbonates | superoxide dismutase 1 | Homo sapiens |