Title : The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions.

Pub. Date : 2005 Apr

PMID : 15766740






2 Functional Relationships(s)
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1 DESIGN AND METHODS: After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A2 forms of NAG were separated by ion-exchange chromatography on DEAE cellulose. DEAE-Cellulose O-GlcNAcase Homo sapiens
2 CONCLUSIONS: The determination of the denoted isoenzymes of urinary NAG after ultrafiltration, dialysis, and chromatographic separation on DEAE cellulose is reliable in a wide range of alkaline pH values of urine. DEAE-Cellulose O-GlcNAcase Homo sapiens