Title : Metformin-induced suppression of glucose-6-phosphatase expression is independent of insulin signaling in rat hepatoma cells.

Pub. Date : 2005 Mar

PMID : 15702236






5 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Metformin-induced suppression of glucose-6-phosphatase expression is independent of insulin signaling in rat hepatoma cells. Metformin glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus
2 To elucidate the pharmacological action of metformin on hepatic glucose production, we examined its effect on the gene expression of glucose-6-phosphatase (G6Pase), a key enzyme of gluconeogenesis, in H4IIE rat hepatoma cell line by RT-PCR and quantitative real-time PCR. Metformin glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus
3 To elucidate the pharmacological action of metformin on hepatic glucose production, we examined its effect on the gene expression of glucose-6-phosphatase (G6Pase), a key enzyme of gluconeogenesis, in H4IIE rat hepatoma cell line by RT-PCR and quantitative real-time PCR. Metformin glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus
4 Metformin suppressed dexamethasone/cAMP-induced expression of G6Pase mRNA in a dose dependent manner, its maximum effect being observed at 2 mM (79.3% inhibition, P<0.05). Metformin glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus
5 In the present study, we demonstrate that metformin suppresses G6Pase mRNA expression by a mechanism that is independent of the activation of PI3-kinase, Akt, MAP kinase and protein kinase C pathway in hepatocytes. Metformin glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus