Title : Upregulation of neuronal nitric oxide synthase mRNA and protein in adrenal medulla of water-deprived rats.

Pub. Date : 2005 Jan

PMID : 15637337






5 Functional Relationships(s)
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1 Upregulation of neuronal nitric oxide synthase mRNA and protein in adrenal medulla of water-deprived rats. Water nitric oxide synthase 1 Rattus norvegicus
2 Using an RT-PCR technique, the relative quantities of nNOS mRNA as well as the tyrosine hydroxylase and phenylethanolamine N-methyltransferase mRNA in the adrenals of water-deprived rats significantly increased from 12 hr to 4 days. Water nitric oxide synthase 1 Rattus norvegicus
3 In situ hybridization and immunohistochemical study showed that water deprivation activated nNOS mRNA and protein expression in the adrenal medulla. Water nitric oxide synthase 1 Rattus norvegicus
4 Four days after water deprivation, nNOS protein expression determined by Western blot significantly increased in the adrenal gland. Water nitric oxide synthase 1 Rattus norvegicus
5 Our results are the first to demonstrate that nNOS syntheses in the adrenal medulla are markedly increased in water-deprived rats. Water nitric oxide synthase 1 Rattus norvegicus