Title : Glucose stimulates the expression and activities of nitric oxide synthases in incubated rat islets: an effect counteracted by GLP-1 through the cyclic AMP/PKA pathway.

Pub. Date : 2005 Feb

PMID : 15558323






4 Functional Relationships(s)
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1 Here, we show that glucose stimulation (20 mmol/l) induces iNOS and increases ncNOS activity. Glucose nitric oxide synthase 2 Rattus norvegicus
2 The addition of glucagon-like-peptide 1 (GLP-1) or dibutyryl-cAMP to islets incubated with 20 mmol/l glucose results in a marked suppression of iNOS expression and activity, a reduction in ncNOS activity and increased insulin release. Glucose nitric oxide synthase 2 Rattus norvegicus
3 The GLP-1-induced suppression of glucose-stimulated iNOS activity and expression and its stimulation of insulin release is, at least in part, PKA dependent, since the PKA inhibitor H-89 reverses the effects of GLP-1. Glucose nitric oxide synthase 2 Rattus norvegicus
4 These observations have been confirmed by confocal microscopy showing the glucose-stimulated expression of iNOS, its suppression by GLP-1 and its reversion by H-89 in beta-cells. Glucose nitric oxide synthase 2 Rattus norvegicus