Title : Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals.

Pub. Date : 2004 Aug 17

PMID : 15284440






1 Functional Relationships(s)
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1 Fused to the rapamycin-binding domain (FRB) of the kinase mammalian target of rapamycin and FK506-binding protein 12 (FKBP), respectively, the optimized FRB-N-terminal luciferase fragment (NLuc)/C-terminal luciferase fragment (CLuc)-FKBP luciferase complementation imaging (LCI) pair reconstituted luciferase activity in cells upon single-site binding of rapamycin in an FK506-competitive manner. Sirolimus FKBP prolyl isomerase 1A Homo sapiens