Title : Measurement of agonist-stimulated [35S]GTPgammaS binding to assess total G-protein and Galpha-subtype-specific activation by G-protein-coupled receptors.

Pub. Date : 2004

PMID : 15250494






1 Functional Relationships(s)
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1 In membrane preparations, GTP can be substituted by 35S-labeled guanosine 5"-O-(3-thio)triphosphate ([35S]GTPgammaS) and on agonist stimulation a stable [35S]GTPgammaS-Galpha complex will form and accumulate. Guanosine 5'-O-(3-Thiotriphosphate) succinate-CoA ligase GDP/ADP-forming subunit alpha Homo sapiens