Title : Kinetic analysis of a model for double substrate cycling: highly amplified ADP (and/or ATP) quantification.

Pub. Date : 2004 Jun

PMID : 15189857






1 Functional Relationships(s)
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1 The model is illustrated by the quantification of nanomolar levels of ADP (and/or ATP) in a continuous assay involving the enzymes L-lactate dehydrogenase and L-lactate oxidase to cycle the pyruvate accumulated in a first enzymatic cycle constituted by the enzymes pyruvate kinase and hexokinase. Adenosine Diphosphate hexokinase 1 Homo sapiens