Title : Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris.

Pub. Date : 2004 Mar

PMID : 14693913






1 Functional Relationships(s)
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1 Using hBSSL as a platform to study Pichia"s O-glycosylation capabilities, we found that nearly all of these sites were occupied by mannose-containing O-glycans, whose structures, after beta-elimination and purification, were assigned by (1)H NMR and, in some cases, by linkage-specific exoglycosidases and methylation analysis. o-glycans carboxyl ester lipase Homo sapiens