Title : A novel non-radioactive method for detection of nucleoside analog phosphorylation by 5'-nucleotidase.

Pub. Date : 1992 Aug

PMID : 1430786






2 Functional Relationships(s)
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Sentence
Compound Name
Protein Name
Organism
1 In vitro, IMP and GMP serve as the optimal phosphate donors for this nucleoside phosphotransferase reaction. Phosphates inositol monophosphatase 1 Homo sapiens
2 However, detection of the phosphorylation of unlabeled nucleoside by HPLC is difficult since the ultraviolet absorbance of the phosphate donor, IMP, frequently obscures the absorbance of newly formed nucleotide. Phosphates inositol monophosphatase 1 Homo sapiens