Title : Studies on pH regulatory mechanisms in cultured astrocytes of DBA and C57 mice.

Pub. Date : 1992 Sep-Oct

PMID : 1396416






6 Functional Relationships(s)
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1 It also enhanced the response of pHi to changing pHo and [Na+]o in HEPES HBSS. hbss glucose-6-phosphate isomerase 1 Mus musculus
2 In nominal HCO3(-)-free HEPES-buffered Hanks" balanced salt solution (HEPES HBSS), when the pH of medium (pHo) was maintained at 7.4, the steady-state pHi of cultured astrocytes from DBA mice was 6.98 +/- 0.03, and that from C57 mice was 7.01 +/- 0.03. hbss glucose-6-phosphate isomerase 1 Mus musculus
3 When the cells were incubated in HBSS containing 25 mM HCO3- and equilibrated with 5% CO2 (HCO3- HBSS, pHo = 7.4), pHi of both DBA and C57 astrocytes was approximately 0.1-0.15 pH units higher than that in HEPES HBSS. hbss glucose-6-phosphate isomerase 1 Mus musculus
4 When the cells were incubated in HBSS containing 25 mM HCO3- and equilibrated with 5% CO2 (HCO3- HBSS, pHo = 7.4), pHi of both DBA and C57 astrocytes was approximately 0.1-0.15 pH units higher than that in HEPES HBSS. hbss glucose-6-phosphate isomerase 1 Mus musculus
5 When the cells were incubated in HBSS containing 25 mM HCO3- and equilibrated with 5% CO2 (HCO3- HBSS, pHo = 7.4), pHi of both DBA and C57 astrocytes was approximately 0.1-0.15 pH units higher than that in HEPES HBSS. hbss glucose-6-phosphate isomerase 1 Mus musculus
6 SITS, an anion transport inhibitor, decreased the pHi of both DBA and C57 astrocytes in HCO3- HBSS but not in HEPES HBSS. hbss glucose-6-phosphate isomerase 1 Mus musculus