Title : Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.

Pub. Date : 2003 Aug 19

PMID : 12909725






1 Functional Relationships(s)
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1 We demonstrate that our error-prone Pol I can be used to generate enzymes with distinct properties by generating TEM-1 beta-lactamase mutants able to hydrolyze a third-generation lactam antibiotic, aztreonam. Aztreonam TEM-1 beta-lactamase Escherichia coli