Title : Iron-sulfur cluster biosynthesis. Characterization of frataxin as an iron donor for assembly of [2Fe-2S] clusters in ISU-type proteins.

Pub. Date : 2003 May 21

PMID : 12785837






4 Functional Relationships(s)
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1 The intermediate [2Fe-2S] ISU-bound cluster is formed by delivery of iron and sulfur to the apo ISU, with the latter delivered through an IscS-mediated reaction. Sulfur NFS1 cysteine desulfurase Homo sapiens
2 2002, 124, 8774-8775] that this iron-bound form of ISU is viable for assembly of holo ISU, either by subsequent addition of sulfide or by NifS-mediated sulfur delivery. Sulfur NFS1 cysteine desulfurase Homo sapiens
3 With NifS as a sulfur donor, yields in excess of 70% of holo ISU were obtained. Sulfur NFS1 cysteine desulfurase Homo sapiens
4 Similar rates were obtained for NifS-mediated sulfur delivery, consistent with iron release from frataxin as a rate-limiting step in the cluster assembly reaction. Sulfur NFS1 cysteine desulfurase Homo sapiens