Title : Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species.

Pub. Date : 2003 Jan

PMID : 12693981






1 Functional Relationships(s)
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1 Highly purified cytochromes P45017alpha were used for determination of enzyme activity and specificity in relation to progesterone, pregnenolone, 17alpha-hydroxyprogesterone, and 17alpha-hydroxypregnenolone with registration of the kinetics of reaction product formation using HPLC. Progesterone cytochrome P450, family 17, subfamily a, polypeptide 1 Rattus norvegicus