Title : Resveratrol-induced G2 arrest through the inhibition of CDK7 and p34CDC2 kinases in colon carcinoma HT29 cells.

Pub. Date : 2003 Apr 1

PMID : 12663041






5 Functional Relationships(s)
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1 Resveratrol-induced G2 arrest through the inhibition of CDK7 and p34CDC2 kinases in colon carcinoma HT29 cells. Resveratrol cyclin dependent kinase 1 Homo sapiens
2 Western blot analysis and kinase assays demonstrated that the perturbation of G(2) phase progression by resveratrol was accompanied by the inactivation of p34(CDC2) protein kinase, and an increase in the tyrosine phosphorylated (inactive) form of p34(CDC2). Resveratrol cyclin dependent kinase 1 Homo sapiens
3 Western blot analysis and kinase assays demonstrated that the perturbation of G(2) phase progression by resveratrol was accompanied by the inactivation of p34(CDC2) protein kinase, and an increase in the tyrosine phosphorylated (inactive) form of p34(CDC2). Resveratrol cyclin dependent kinase 1 Homo sapiens
4 Kinase assays revealed that the reduction of p34(CDC2) activity by resveratrol was mediated through the inhibition of CDK7 kinase activity, while CDC25A phosphatase activity was not affected. Resveratrol cyclin dependent kinase 1 Homo sapiens
5 In addition, resveratrol-treated cells were shown to have a low level of CDK7 kinase-Thr(161)-phosphorylated p34(CDC2). Resveratrol cyclin dependent kinase 1 Homo sapiens