Title : A new ligand for an old lipocalin: induced circular dichroism spectra reveal binding of bilirubin to bovine beta-lactoglobulin.

Pub. Date : 2003 Mar 27

PMID : 12650931






1 Functional Relationships(s)
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1 Due to intramolecular chiral exciton coupling between the dipyrrinone chromophores, the long-wavelength negative and short-wavelength positive CD bands clearly prove that a single bilirubin molecule binds to BLG in a left-handed helical conformation (in pH 7.4 phosphate buffer Deltaepsilon(min) is -54 M(-1) cm(-1) at 467 nm and Deltaepsilon(max) is +48.5 M(-1) cm(-1) at 412 nm). Phosphates beta-lactoglobulin Bos taurus