Pub. Date : 2003 Feb 21
PMID : 12468550
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | New insights into the tPA-annexin A2 interaction. | Tetradecanoylphorbol Acetate | annexin A2 | Homo sapiens |
| 2 | The annexin A2 motif that mediates tissue plasminogen activator interaction has been assigned to the hexapeptide LCKLSL in the amino-terminal domain of the protein, and it has been proposed that Cys(8) of this sequence is essential for tPA binding. | Tetradecanoylphorbol Acetate | annexin A2 | Homo sapiens |
| 3 | In an attempt to identify other amino acids critical for tPA-annexin A2 interaction, we have analyzed a set of peptides containing several modifications of the original hexapeptide, including glycine scans, alanine scans, d-amino acid scans, conservative mutations, cysteine blocking, and enantiomer and retroenantiomer sequences. | Tetradecanoylphorbol Acetate | annexin A2 | Homo sapiens |
| 4 | Using a non-radioactive competitive binding assay, we have found that all cysteine-containing peptides, independently of their sequence, compete the interaction between tPA and annexin A2. | Tetradecanoylphorbol Acetate | annexin A2 | Homo sapiens |
| 5 | These data call for a revision of the role of the LCKLSL sequence as the sole annexin A2 structural region required to bind tPA and indicate that further studies are necessary to better define the annexin A2-tPA interaction. | Tetradecanoylphorbol Acetate | annexin A2 | Homo sapiens |