Title : Preferential DNA damage and poor repair determine ras gene mutational hotspot in human cancer.

Pub. Date : 2002 Oct 16

PMID : 12381705






1 Functional Relationships(s)
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1 METHODS: We used the UvrABC nuclease incision method in combination with ligation-mediated polymerase chain reaction to map the distribution of DNA adducts induced by benzo[a]pyrene diol epoxide (BPDE) and other bulky carcinogens within exons 1 and 2 in H-ras, N-ras, and K-ras. 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide KRAS proto-oncogene, GTPase Homo sapiens